Exciting developments in next generation sequencing, microfluidics, and microscopy have spurned an era of new technologies to measure gene expression in individual cells and in tissues.  I will discuss our technological contributions—in the space of single-cell gene expression analysis, as well as a new technology we developed, in collaboration with Fei Chen’s lab, called Slide-seq, which quantifies genome-wide expression at 10 micron spatial resolution.  I’ll also highlight some areas of biology in which we are particularly focused on deploying these new tools.